Figure 1.

Bead based screening of HCR detection schemes. (a) Streptavidin‐coated beads immobilize the biotinylated initiator to start the hybridization chain reaction in the presence of H1‐Cy5 and H2. (b) Flow cytometry analysis of the best (left) and the worst (right) designed oligonucleotides after 30 min and 24 h of hybridization at 4 °C. The time‐dependent increase in fluorescence with N10 L6 shows the amplification of the signal in comparison with H1 alone. Histograms are normalized to 100 %. (c) Screening of ten oligonucleotides by flow cytometry after 30 min, 2 h, 4 h and 24 h of hybridization.