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. 2020 Feb 3;226(3):770–784. doi: 10.1111/nph.16398

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© 2019 The Authors New Phytologist © 2019 New Phytologist Trust

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Dose‐dependent inhibition of H⁺‐ATPase activity measured in Spinacia oleracea plasma membranes (PMs) in response to tenuazonic acid (TeA) treatment. Values are means ± SEM. Experiments with Stemphylium loti fractions 1.9 and 1.10 (a), and 2.6 and 2.7 (b) were carried out in triplicate (n = 3), whereas experiments with TeA (c) were carried out in triplicate and on three independent PM fractions (n = 9). (d, e, f) Data were analyzed using nonlinear regression and fitted to log[inhibitor] (µg) vs the normalized response for determination of IC₅₀ values.