Table 4.
IC50 values of tenuazonic acid (TeA) for Spinacia oleracea H+‐ATPase, Saccharomyces cerevisiae PMA1, and truncated Arabidopsis thaliana H+‐ATPases aha2Δ92 and aha2Δ40.
| IC50 (μM) | |
|---|---|
| Spinacia oleracea H+‐ATPase | 0.68 (0.59–0.78) |
| AHA2 (expressed in yeast) | 14.33 (12.0‐17.1) |
| PMA1 | 7.8 (5.8–10.4) |
| aha2Δ92 | ND |
| aha2Δ77 | ND |
| aha2Δ40 | 3.7 (2.7–5.0) |
ATP hydrolysis of plasma membrane (PM) fractions from S. oleracea and S. cerevisiae expressing PMA1, aha2Δ92, aha2Δ77 and aha2Δ40 or endoplasmic reticulum (ER) enriched fraction with AHA2, all treated with increasing concentrations of TeA. Data were analyzed using nonlinear regression and fitted to log[TeA] vs the response (variable to slope) (bottom constrain = 0.0) to determine the IC50 values. ND, not determined as data could not be fitted to the model.