Table 1.
Post‐translational modifications of myosin in response to long‐term mechanical and immobilization with and without BGP‐15 treatment
| Group comparison | Modification, position | Found in | Confirmation MS/MSa,b |
|---|---|---|---|
| Group ‘5 days, no drug' vs control group (in all 5 days, no drug but also in 1‐2 of the controls) | Acetylation, 83 K | 2 out of 6 control samples | NO |
| Methylation, 1372 R | 1 out of 6 control samples | YES | |
| Methylation, 1749 R | 2 out of 6 control samples | YES | |
| Oxidation, 826 N | 2 out of 6 control samples | NO | |
| Oxidation, 1720 N | 2 out of 6 control samples | YES | |
| Group ‘5 days, no drug' vs group ‘5 days + BGP‐15' (in all 5 days, no drug but also in 1 of 5 days, BGP‐15 treated) | Methylation, 1372 R | 1 out of 4 samples of group ‘5 days + BGP‐15' | YES |
| Oxidation, 826 N | 1 out of 4 samples of group ‘5 days + BGP‐15' | NO | |
| Oxidation, 1771 K | 1 out of 4 samples of group ‘5 days + BGP‐15' | NO | |
| Oxidation, 1774 D | 1 out of 4 samples of group ‘5 days + BGP‐15' | YES | |
| Group ‘5 days + BGP‐15' vs control group (in all 5 days + BGP‐15 but also in 1‐2 of controls except when unique) | Acetylation, 72 K | 1 out of 6 control samples | NO |
| Methylation, 1749 R | 2 out of 6 control samples | YES | |
| Oxidation, 490 N | Unique | YES | |
| Oxidation, 1234 N | 1 out of 6 control samples | NO | |
| Group ‘5 days + BGP‐15' vs group ‘5 days, no drug' | Acetylation, 680 S | Unique | NO |
a
Oxidation, 1774 D, Oxidation, 490 N were confirmed using MaxQuant.
b
Oxidation, 1720 N was confirmed using both MaxQuant and PD.