Figure 8.

Virus‐like particle (VLP) levels produced from a Gag expressor alone are not altered upon overexpression of EAP45 in HAP1 EAP45 KO cells. (a): EAP45 KO cells were transfected with WT provirus or a Gag expressor in the absence or presence of EAP45. Levels of protein from cell lysates or purified virions were detected by western blot using antibodies specific to p24 or GAPDH. (b): The VLP release ratio at each condition was quantified by densitometry and normalised to that in the absence of EAP45 expressor. The error bars represent the standard error of the mean of four replicates from three independent experiments. (c): The densitometric traces of p24 and p24‐p2 for WT provirus or p55 for a Gag expressor alone are shown separately or in overlay