Table 1.
Taxonomic affiliation and statistics of key amplicon sequence variants (ASVs). Supplementary Data S1 lists taxonomic classifications, sequences and abundances of all ASVs across all samples.
| Physiology | Genus | Class | # ASVs obser‐ved | Field average abun‐dance (%) | Mesocosm average abundance (%) | Mesocosm maximum abundance (%) | #observations | ASVs |
|---|---|---|---|---|---|---|---|---|
| Methanotroph | Methylocystis/sinusa | Alpha‐proteobacteria | 3 | 0.0 | 2.4 | 32.3 | 196 | 15, 23, 82 |
| Methanotroph | Methylobacter | Gamma‐proteobaceria | 5 | 14.5 | 0.1 | 6.1 | 56 | 55, 124, 272, 444, 1051 |
| Methanotroph | Methylovulum | Gamma‐proteobaceria | 6 | 0.4 | 0.4 | 19.8 | 153 | 34, 40, 167, 402, 592, 1057 |
| Methanotroph | Methylomonas | Gamma‐proteobaceria | 2 | 0.0 | 0.1 | 24.0 | 19 | 89, 187 |
| Methanotroph | Crenothrix | Gamma‐proteobaceria | 2 | 0.0 | 0.0 | 0.8 | 11 | 437, 823 |
| Methylotroph | Hyphomicro‐bium | Alpha‐proteobacteria | 8 | 0.0 | 0.2 | 4.6 | 113 | 88, 228, 424, 853, 1115, 1227, 1831, 3223 |
| Methylotroph | Methylo‐bacterium | Alpha‐proteobacteria | 3 | 0.0 | 0.0 | 1.0 | 24 | 533, 710, 875 |
| Methylotroph | Methylo‐versatilis | Beta‐proteobacteria | 1 | 0.0 | 2.1 | 29.4 | 194 | 14 |
| Methylotroph | Methylotenera | Beta‐proteobacteria | 6 | 2.1 | 0.2 | 3.6 | 122 | 106, 190, 327, 539, 646, 945 |
| Methylotroph | Methylophilus | Beta‐proteobacteria | 3 | 3.0 | 0.1 | 13.5 | 14 | 111, 153, 1552 |
| Unknown | Gracilibacteria | Candidate Phyla Radiation | 2 | 0.0 | 0.6 | 12.1 | 150 | 24, 74 |
| Fermentation | Pelosinus | Negativicutes | 11 | 0.0 | 2.3 | 86.4 | 94 | 10, 19, 707, 1452, 1592, 2442, 2550, … |
| Sulfate reduction | Desulfosporosinus | Clostridia | 40 | 2.4 | 0.3 | 19.8 | 162 | 46, 351, 374, 381, 382, 457, 525, 724, … |
a
These two genera could not be discriminated based on the 400 nucleotide amplicon.