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. 2020 Feb 27;22(4):1356–1369. doi: 10.1111/1462-2920.14947

Figure 1.

Figure 1

Determination of polyester degradation by Alcanivorax sp. 24.A. Clear zone hydrolysis test of Alcanivorax sp. 24 on the five aliphatic polyesters PHB, PHBV, PES, PBS and PCL. Polyesters were added at 0.3% w/v to BH mineral media containing 1% agarose (w/v). Arrows highlight the hydrolysis halos surrounding the 5 mm‐diameter wells made for Alcanivorax sp. 24 inoculation.B. Growth curves of Alcanivorax sp. 24 when incubated in the presence of three different polyesters, succinate (labile substrate control) and BH mineral media (MM; negative control). Increase in biological biomass was assessed by protein quantification. Error bars indicate the standard deviation of three biological replicates.C. Degradation of the PET intermediate BHET by Alcanivorax sp. 24. The substrate BHET and its hydrolysed product TPA were monitored by LC–MS. ‘No inoculum’ represents the replicate control condition where Alcanivorax sp. 24 was not inoculated. [Color figure can be viewed at http://wileyonlinelibrary.com]