Figure 9.

Western blot analyses of Na⁺, K⁺ ATPase α1 and phospholemman between animal groups

A, western blot of Na⁺,K⁺‐ATPase (NKA) α1 expression in Sham and AC groups at 60 and 150 days post‐operatively. The Na⁺,K⁺‐ATPase α1 relative expression was significantly reduced in 60 and 150 days compared to their age‐matched Sham groups. N = 5 for 60Sham and N = 4 for 60AC; N = 6 for 150Sham, N = 7 for 150AC. Mean (SD). Student's t test one‐tailed, ^* P < 0.05, ^** P < 0.01. B, western blot of expression of phospholemman (PLM), a protein which regulates the activity of Na⁺,K⁺‐ATPase. The relative expression of PLM significantly decreases between 60‐day sham and 60‐day AC, but was unchanged between the 150‐day AC group compared to age‐matched Sham. Mean (SD). Student's t test one‐tailed, ^* P < 0.05. N = 5 for 60Sham and N = 7 for 60AC; N = 4 for 150Sham, N = 4 for 150AC.