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. 2020 Feb 26;59(15):6192–6195. doi: 10.1002/anie.201914449

Figure 4.

Figure 4

Creation of a luxRC from PseR for activation of a silent BGC. The LasR SBD was fused with the PseR DBD in the pMQ72 vector backbone and introduced into WT SZ57. The luxRC was induced with 100 mm arabinose and activated by adding 10 μm 3‐oxo‐C12 HSL. When cultured in in LB medium, SZ57 produces syringafactins A and C [peaks 2 in the HPLC spectra], and no other significant lipophilic secondary metabolites are detected. With LuxRC, an additional metabolite was produced [peak 1 in spectrum (i)]. UV detection was at λ=210 nm.