Table 1.

Results of Simplex-Centroid experimental design for total phenols and flavonoids, EC₅₀, HEp-2 cell line and mononuclear cells

Solvents				Response variables
Extract	H2O	EtOH	MeOH	TPC mgGAc/g	TFC mgQ/g	EC50 μg/mL	HEp-2 (%)	MVC (%)
1	0.0	50.0	50.0	339.52 ± 0.01	130.22 ± 0.02	3.85 ± 0.01	104.46 ± 0.08	214.92 ± 3.88
2	0.0	100.0	0.0	344.12 ± 0.01	128.46 ± 0.01	3.39 ± 0.01	189.90 ± 0.33	196.41 ± 18.27
3	16.7	16.7	66.6	322.78 ± 0.01	119.69 ± 0.01	4.97 ± 0.01	151.30 ± 0.04	176.45 ± 1.45
4	100.0	0.0	0.0	285.66 ± 0.01	101.86 ± 0.01	4.41 ± 0.00	74.70 ± 0.10	151.02 ± 10.00
5	0.0	100.0	0.0	412.59 ± 0.01	132.63 ± 0.03	4.13 ± 0.00	54.60 ± 0.17	232.33 ± 17.96
6	0.0	0.0	100.0	330.33 ± 0.01	137.43 ± 0.02	7.71 ± 0.00	43.37 ± 0.19	213.67 ± 11.50
7	50.0	50.0	0.0	318.51 ± 0.02	107.39 ± 0.01	8.38 ± 0.00	54.33 ± 0.30	202.03 ± 4.24
8	100.0	0.0	0.0	268.92 ± 0.00	122.76 ± 0.01	5.34 ± 0.00	63.24 ± 0.15	163.96 ± 4.79
9	16.7	66.7	16.7	312.27 ± 0.01	118.16 ± 0.02	7.58 ± 0.01	65.74 ± 0.39	148.28 ± 5.69
10	0.0	50.0	50.0	335.75 ± 0.01	131.32 ± 0.04	7.43 ± 0.01	98.20 ± 0.10	228.28 ± 3.56
11	66.6	16.7	16.7	322.12 ± 0.02	103.22 ± 0.00	5.71 ± 0.01	80.51 ± 0.04	169.66 ± 0.68
12	33.3	33.3	33.3	299.95 ± 0.03	118.68 ± 0.03	5.47 ± 0.01	71.51 ± 0.29	204.27 ± 3.09
13	50.0	0.0	50.0	164.15 ± 0.02	122.76 ± 0.00	5.00 ± 0.00	94.47 ± 0.14	290.06 ± 13.60
14	0.0	0.0	100.0	324.25 ± 0.02	127.59 ± 0.02	4.25 ± 0.00	121.87 ± 0.14	236.67 ± 20.37

GAc Gallic Acid, Q quercetin