TABLE 1.

Inhibition of HIV gp120 variants by Grft

Straina	Mutation	Fold differenceb		Pc
		Mean	SE
PVO.4
Single mutants	−197	+2.7	0.58	1.6E−06
	+230	+2.1	0.56	0.005
	−234	+2.2	0.58	3.7E−06
	−241	+3.8	0.91	7.2E−09
	−289	+2.8	0.79	0.001
	−295	+11.1	5.83	< 2.2e−16
	−301	+1.4	0.32	6.6E−04
	−332	+4.6	1.22	1.4E−08
	−339	+2.8	0.59	3.5E−11
	−386	+1.5	0.73	0.5
	−392	+1.7	0.35	4.5E−05
	−448	+5.1	0.91	9.7E−12
Double and triple mutants	−234, −295	+9.8	3.2	1.7E−07
	−234, −448	+6.4	2.4	9.2E−10
	−295, −332	+42.9	6.9	2.2E−16
	−295, −448	+23.2	7.8	2.7E−10
	−332, −448	+35.5	7.1	9.4E−07
	−234, −295, −448	+28.2	5.0	3.15E−13
	−295, −332, −448	+88.2	10.7	8.1E−14
Shift of glycosylation site mutants	332 > 334	+1.3	0.49	0.2
	448 > 446	+1.7	0.79	0.04
	−295, 332 > 334	+8.0	2.3	8.1E−09
	−295, 448 > 446	+3.2	0.9	6.6E−09
	332 > 334, −448	+6.6	2.7	1.1E−07
	−332, 448 > 446	+10.2	3.3	1.4E−11
	332 > 334, 448 > 446	+5.4	3.7	8.8E−05
	−295, 332 > 334, −448	+21.2	18.0	3.8E−05
	−295, −332, 448 > 446	+29.4	11.5	4.3E−09
	−295, 332 >334, 448 >446	+21.5	4.7	2.8E−06
BaL.01	−295	+14.1	1.9	1.0E−10
	−332	+2.1	0.6	0.002
	−448	+1.0	0.3	0.57
ZM109	+234	−10.2	2.6	1.0E−11
	+295	−52.9	12.6	8.1E−13
	+332	−1.0	0.3	0.8
	−334	+1.4	0.2	0.005
	+446	+1.2	0.1	0.03
	−448	−4.3	1.2	1.4E−11
	+234, +295	−157	66	2.6E−16
	+234, +446	−20.6	6.3	1.1E−11
CAP45	−234	+1.2	0.3	0.4
	+295	−3.2	1.2	7.3E−10
	+332	−3.2	1.2	4.7E−08
	−334	−2.3	0.9	1.1E−03
	−446	+2.0	0.9	1.5E−03
	+448	−1.3	0.6	0.1

^aThe Gfrt IC₅₀ for wild-type strains PVO.4, BaL.01, ZM109, and CAP45 are 0.2 ± 0.09, 0.26 ± 0.08, 26.9 ± 6.2, and 0.54 ± 0.24 nM (standard error), respectively.

^bMean is the average of triplicate data replicated a minimum of three times. A plus sign indicates that Grft inhibits the wild-type virus better than the mutant virus. A minus sign indicates that Grft inhibits the wild-type virus less well than the mutant virus (the mutant is more sensitive to Grft).

^cP values were determined by an analysis of variance with the Bonferroni correction.