TABLE 4.
Mutations identified in mutants isolated during serial passage in CHIR-090
| Straina | Passage step (CHIR-090 MIC in μg/ml) | Mutation or amino acid substitution(s) |
|---|---|---|
| K2732 (WT efflux) | Parent (1) | |
| K2732-P2 | 2 (4) | FabF1T306A |
| K2732-P6 | 6 (32) | nfxB stop TGA-Cys; FabGD190G; PA4465N193T |
| K2732-P13 | 13 (64) | MexRT130P; nfxB stop TGA-Cys; FabGD190G; PA4465N193T |
| K2733 (efflux deficient) | Parent (0.0313) | |
| K2733-P6a (nc) | 6 (0.25) | PA4465N193T |
| K2733-P6b (sc) | 6 (0.5) | FabGA159V |
| K2733-P13a (nc) | 13 (0.5) | FabGA167V; PA4465N193T |
| K2733-P13b (sc) | 13 (1) | FabGA167V; PA4465N193T |
a
nc, normal colony size; sc, small colony size.