TABLE 3.
Annotation of the putative mobile genetic elements (MGE) in which cfr-like genes were detectedc
| Isolate(s) | Element syntenyc | Cfr type | MGE type | Genome insertion site |
|---|---|---|---|---|
| HON06 HON11 | Transposase, excisionase, replication initiation factor, transcriptional regulator, methyltransferase, HTH-type transcriptional regulator, hypothetical protein, Cfr-like protein, MATE efflux protein, RNA polymerase sigma factor, HTH-domain containing protein, hypothetical protein, HTH-type transcriptional regulator, Hypothetical protein | Cfr(B) | Tn6218-like transposona | Between genes for a hypothetical protein and an HTH transcriptional regulator |
| PUC51 PUC347 | Transposase, Cfr-like protein, integrase, RNA methylase, hypothetical protein, endonuclease, hypothetical protein, mobilization protein, helicase | Undescribed | ||
| HON10 LIBA5707 | Resolvase, resolvase, hypothetical protein, hypothetical protein, RNA polymerase sigma factor, Cfr-like protein, hypothetical protein, hypothetical protein, transcriptional regulator, HTH transcriptional regulator, relaxase | Cfr(C) | F548-like ICEb | Gene for ABC transporter permease |
| DF11 | DNA invertase, recombinase, hypothetical protein, N-acetyltransferase, ABC transporter ATP binding protein, Cfr-like protein, HTH transcriptional regulator, hypothetical protein | Cfr(E) | Undescribed | Gene for adenine deaminase adeC |
a
GenBank accession number for Tn6218 in C. difficile Ox2167, HG002396.
b
GenBank accession number for C. difficile F548 assembly, GCA_000452325.2 (ASM45232v2).
c
HTH, helix-turn-helix; MATE, multiantimicrobial extrusion.