FIG 4.

High Sle1 activity reduces the cell size, while oxacillin increases cell size. The cell volume was estimated for JE2 wild-type, JE2clpX_I265E, JE2sle1, and JE2clpX_I265E,sle1 cells growing exponentially at 37°C in the absence of oxacillin (A) or after exposure to 0.05 μg ml⁻¹ oxacillin for 20 min (B). Cells were stained with the membrane dye Nile Red before imaging by SR-SIM. For each strain, the cell volume was determined in 300 cells representing 100 cells from each of the three growth phases by fitting an ellipse to the border limits of the membrane. The graphs represent data from two biological replicates.