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Staining and laser specifications used for SR-SIM
| Staining | Concn | Target | Laser (nm) | Laser type, mW | Laser power (%/ms) | Beam splitter | Grating (μm) |
|---|---|---|---|---|---|---|---|
| Nile Red | 5 μg ml−1 | Membrane | 561 | HR diode, 100 | 5/100 | BP 570-650 + LP 750 | 34 |
| HADA | 250 μM | New PG | 405 | HR diode, 50 | 20/100 | BP 420-480 + LP 750 | 23 |
| WGA-488 | 1 μg ml−1 | Old PG | 488 | HR diode, 100 | 5/100 | BP 495-575 + LP 750 | 28 |
