Figure 5. Elution profiles of pools of C. durissus venom by RP-HPLC monitored by 215 nm. Samples of 3 mg of lyophilized venom pools were dissolved in 0.1% trifluoroacetic acid (TFA) (solution A) and subjected to RP-HPLC on a C18 column. Elution was performed at 1.0 mL/min by applying a gradient toward 0.1% TFA and 95% acetonitrile (solution B), as described in the experimental section. Red (+): crotamine-positive venom pool; blue (-): crotamine-negative venom pool.