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. 2020 Apr 21;11:189. doi: 10.3389/fendo.2020.00189

Figure 2.

Figure 2

The overexpression of IDH1 promoted cell proliferation of cholangiocarcinoma. (A) Western blot was used to determine the expressions of IDH1 in the IDH knockout (KO) REB cells. Cell count assay (B) and MTT assay (C) were used to determine the cell viabilities of REB and IDH1 KO REB cells. (D) qPCR was used to determine the relative expressions of IDH1 in IDH1 KO REB cells transfected with vector, plasmids containing wildtype IDH1 or plasmids containing IDH1 R132C mutation sequence. Cell count assay (E) and MTT assay (F) were used to determine cell viabilities of IDH1 KO cells transfected with vector, plasmids containing wildtype IDH1 or plasmids containing IDH1 R132C mutation type. (G) The tumor volume of xenograft mouse transplanted with IDH1 KO REB cells transfected with vector, wildtype IDH1 or IDH1 R132C mutation plasmid. (H) Immunohistochemistry demonstrated percentage of Ki67 positive in tumor tissue from each group. Data are shown as mean ± S.D. **P < 0.01, ***P < 0.001; ns indicates no significance.