Figure 4.

IDH1 mutation reduced NADPH and α-KG levels. (A,B) ELISA was used to determine the levels of α-KG in REB cells, IDH1 KO REB cells, IDH1 KO REB cells transfected with vector, wildtype IDH1 or IDH1 R132C mutation plasmid. (C,D) ELISA was used to determine the levels of NADPH in REB cells, IDH1 KO REB cells, IDH1 KO REB cells transfected with vector, wildtype IDH1 or IDH1 R132C mutation plasmid. (E) Cell count assay were used to determine the cell viabilities of REB cells or REB IDH1 K.O. cells treated with or without N-acetyl cysteine. (F) Cell migration and invasion assay of the REB cells or REB IDH1 K.O. cells treated with or without N-acetyl cysteine. Data were shown as mean ± S.D. *P < 0.05, ***P < 0.001; ns indicates no significance.