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. 2020 Apr 21;10:624. doi: 10.3389/fonc.2020.00624

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Copyright © 2020 Zu, Wen, Xu, Li, Mi, Li, Brakebusch, Fisher and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ATF3 overexpression suppressed expression of pro-inflammatory cytokines IL-6 and IL-8 in HDFs. (A) The mRNA levels of cytokine, growth factor and CAF-related genes were measured by qRT-PCR in ATF3-OE HDFs and control NEO HDFs. (B) The mRNA levels of the same genes as in (A) were measured by qRT-PCR in two HDF pools with CRISPR/Cas9-mediated ATF3 deletion by independent small guide RNAs (ATF3-SG1 or ATF3-SG2) and compared with the mRNA levels in control HDFs transduced with empty vector (CON-V). (C,D) Protein concentrations of IL-6, IL-8 and TNFα measured by ELISA in CM derived from ATF3-OE or control NEO HDFs (C) and in CM from HDFs with CRISPR/Cas9-mediated deletion of ATF3 (ATF3-SG1 or ATF3-SG2) or control vector (CON-V) (D). Data are presented as the mean ± standard deviation, *p < 0.05, **p < 0.01.