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. 2020 Apr 21;10:624. doi: 10.3389/fonc.2020.00624

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Copyright © 2020 Zu, Wen, Xu, Li, Mi, Li, Brakebusch, Fisher and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ATF3-overexpressing HDFs inhibit melanoma cell proliferation and migration through regulation of the IL-6/STAT3 pathway. (A–F) CM derived from either ATF3-OE or control NEO HDFs, or from ATF3-OE HDFs incubated with 10 ng/ml recombinant human IL-6 (ATF3-OE + rhIL-6) were used to culture melanoma cell lines Mel-JuSo and UACC62 for analysis of growth (A,B), colony formation (C), transwell-migration (D), wound healing (E), and phosphorylated (active) states of the indicated signaling proteins (F). Quantification results for C-F assays are shown in the graphs at right. The relative levels of phosphorylated STAT3 in F were normalized to the respective total STAT3 bands. The scale bars in the images represent 5 mm (C) and 100 μm (D,E). Data are presented as the mean ± standard deviation, *p < 0.05, **p < 0.01.