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. 2020 Apr 24;13:3511–3523. doi: 10.2147/OTT.S238714

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© 2020 Liu et al.

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High glucose induced dysregulated cell function and aberrant expression of MFN1. (A) MFN1 in sections of lung tissues from adjacent normal, LAD and LAD+DM patients. Brown colour indicates positive staining. (B and C). Cell viability was evaluated by the CCK-8 assay in A549 and NCL-H292 cells. (D-E) The protein expression of MFN1 was determined by Western blot analysis in A549 cells (D) and in NCL-H292 cells (E). (F) A549 cells were stained with MFN1 antibody (red) and co-stained with TOM20 antibodies to visualize mitochondria (green) and DAPI staining was used to visualize cell nuclei (blue). Scale bar = 100 μm. Data shown are mean ± SEM, **P < 0.01 (n =6).

Abbreviations: LAD, lung adenocarcinoma; DM, diabetes mellitus; NO, no glucose; LG, low glucose; NG, normal glucose; HG, high glucose. MFN1, mitofusin1; TOM20, mitochondrial import receptor subunit TOM20; DAPI, 4ʹ,6-diamidino-2-phenylindole.