Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 24;13:3511–3523. doi: 10.2147/OTT.S238714

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Liu et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

Glucose enhanced the interaction of MFN1 and Pink in A549 cells. (A) Predicted protein-protein interactions with MFN1 according to the STRING database. (B and C) The expression levels of Pink, Parkin and Fis1 were examined by Western blot in A549 cells after exposure to glucose for 24 hours. (D) Immunofluorescence of A549 cells from NG+NC, HG+NC, and HG+siMFN1. Cells were stained with DAPI (blue) and Pink (red). (E) A549 cells were exposed to 25 mM glucose, and whole cell lysates were extracted for co-immunoprecipitation with anti-Pink, and subsequent probing with anti-MFN1. Scale bar = 100 µm. Data shown are mean ± SEM. **P < 0.01 vs NG+NC group. ^#P < 0.05 vs HG+NC group (n = 4).

Abbreviations: NC, non-targeted control; NG, normal glucose; HG, high glucose. MFN1, mitofusin1; siMFN1, small interfering RNA of MFN1; Pink, PTEN induced kinase; DAPI, 4ʹ,6-diamidino-2-phenylindole. IB, immunoblotting; IP, immunoprecipitation; STRING, https://string-db.org/.