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. 2020 Apr 24;13:3511–3523. doi: 10.2147/OTT.S238714

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© 2020 Liu et al.

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MFN1 mediated the function of A549 cells mainly through Pink. (A and B) Western blot analysis was used to detect the expression of key EMT-related proteins in A549 cells after transfection with MFN1 overexpression plasmid and/or siPink. (C) The survival rate of A549 cells was studied by CCK-8 assay. (D and E) Transwell assays were used to evaluate cell infiltration capacity following overexpression of MFN1 and knockdown of Pink. (F) Representative images are presented to indicate the cellular localization patterns of the mRFP-GFP-LC3 fusion protein; scale bar = 50 μm. Data shown are mean ± SEM. **P < 0.01, ***P < 0.001 vs NG+NC group. ^#P < 0.05, ^##P < 0.01 vs HG+NC group. ^$$P < 0.01, ^$$$P < 0.001 vs HG+MFN1 group (n = 4).

Abbreviations: EMT, epithelial-to-mesenchymal transition; NC, non-targeted control; NG, normal glucose; HG, high glucose. MFN1, mitofusin1; si PINK, small interfering RNA of PTEN induced kinase; LC3B, microtubule-associated proteins 1A/1B light chain 3B; eGFP, enhanced green fluorescent protein; mRFP, monomer red fluorescent protein.