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. 2020 Apr 25;26(6):797–808. doi: 10.1093/ibd/izaa085

FIGURE 2.

FIGURE 2.

Ace2 and Tmprss2 expression in mouse colon and IECs during inflammation. C57Bl/6 mice treated with 3% DSS for indicated times and expression of Ace2, Tmprss2, and IL-1β transcripts in the colon determined by qPCR. A, Body weight variation. N = 7-8 mice; *P < 0.05, ****P < 0.0001 as determined by 2-way ANOVA followed by Sidak posthoc test. B, Gene expression on day 6 of inflammation in whole colon and IECs isolated from colon. N = 7-8 (whole tissue) or n = 5 (colon IECs); **P < 0.01, ***P < 0.001 as determined by unpaired 2-tailed Student t test. C, Time course expression of IL-1β and Ace2 in colonic IECs isolated on days 0, 2, 4, and 6 of DSS treatment. N = 5 mice; IL-1β on day 6 vs day 0, ***P < 0.001; Ace2 on day 4 vs day 0, **P < 0.01; Ace2 on day 6 vs day 0, ***P < 0.001 as determined by 1-way ANOVA followed by Tukey posthoc test. D, Correlation between epithelial Ace2 expression levels and matched colon determinations of MPO (left panel) or epithelial IL-1β (right panel) on days 0, 2, 4, and 6 of DSS. N = 20 mice; Ace2/MPO, r = –0.5623, **P = 0.0099; Ace2/IL-1β, r = –0.6689, **P = 0.0013. E, Expression of Ace2, Tmprss2, and IL-1β in whole colon tissues of wild type and IL-10 KO mice euthanized after development of spontaneous colitis (GSE107810). N = 3 mice; **FDR = 0.0016. F, Expression of Ace2, Tmprss2, and IL-1β in whole colon tissues of recombination-activating gene 1 KO mice injected with regulatory T-cells (control) or naïve T-cells (inflamed). N = 4-6 mice; **FDR = 0.002, ***FDR = 1.95E-5. ANOVA indicates analysis of variance; qPCR, quantitative polymerase chain reaction.