Table 3:

Examples of human thiol-containing proteins involved in the regulation of brain physiology that are targets of E⁺Hg forms. The Hg effects were based on animal models or in vitro studies. The proteins affected in rodents or in cultured cells were selected, and then the human protein information was obtained at www.uniprot.org .

Part A: ENZYMES
Protein	Gene	N° CXXC	N° CXC	N° CC	N° C	N° aa	Function	Localization	Hg effects	Reference
5’-nucleotidase	NT5E	1	0	0	10	574	Nucleotide-binding	Plasma membrane	Inhibition	Sood et al. 1995
Acetylcholinesterase	ACHE	0	0	0	8	614	Neurotransmitter degradation	Plasma membrane	Inhibition	Franciscato et al. 2009; Moraes-Silva et al. 2014
Caspase-8	CASP8	1	0	1	13	479	Apoptosis	Cytoplasm	Activation	Shenker et al. 2002
Complex I (NADH-ubiquinone oxidoreductase 75 kDa subunit)	NDUFS1	3	0	0	18	727	Electron transport	Mitochondrion	Inhibition	Glaser et al. 2010a
Complex II (Succinate dehydrogenase flavoprotein subunit)	SDHA	0	0	2	18	664	Electron transport	Mitochondrion	Inhibition	Glaser et al. 2010a
Complex III (Cytochrome b-c1 complex subunit 1)	UQCRC1	0	0	0	12	480	Electron transport	Mitochondrion	Inhibition	Glaser et al. 2010a
Complex IV (Cytochrome C oxidase subunit 1)	MT-CO1	0	0	0	1	513	Electron transport	Mitochondrion	Inhibition	Glaser et al. 2010a
Cytochrome C	CYCS	1	0	0	2	105	Apoptosis	Mitochondrion	Release	Shenker et al. 1999; 2002
Creatine kinase B-type	CKB	0	0	0	5	381	Transferase	Cytoplasm	Inhibition	Glaser et al. 2010b
Creatine kinase S-type	CKMT2	0	0	0	9	419	Transferase	Mitochondrion	Inhibition	Glaser et al. 2010a
Cystathionine gamma-lyase	CTH	2	0	0	10	405	Cysteine synthesis	Cytoplasm	Inhibition	Bridges et al. 2012
Glutamate-cysteine ligase catalytic subunit	GCLC	0	1	1	14	637	Glutathione synthesis	Cytoplasm	Induction	Thompson et al. 1999
Glutamine synthetase	GLUL	0	0	0	11	373	Glutamine and GABA synthesis	Mitochondrion	Inhibition	Known and Park 2003
Glutathione reductase	GSR	0	0	0	10	522	Glutathione reduction	Mitochondrion	Activation	Stringari et al. 2008; Glaser et al. 2010a; Zemolin et al. 2012
Glutathione S-transferase	GSTA1	0	0	0	1	222	Conjugation of reduced glutathione	Cytoplasm	Activation	Zemolin et al. 2012
Phospholipase A2, membrane associated	PLA2G2A	0	2	2	14	144	Lipid degradation	Plasma membrane	Activation	Mazerik et al. 2007; Verity et al. 1994;
Part B: RECEPTORS
Protein	Gene	N° CXXC	N° CXC	N° CC	N° C	N° aa	Function	Localization	Hg effects	Reference
D(1A) dopamine receptor	DRD1	0	0	1	16	446	G-protein coupled receptor	Plasma membrane, Endoplasmic reticulum membrane	Dysfunction	Daré et al. 2003
D(2) dopamine receptor	DRD2	0	1	0	12	443	G-protein coupled receptor	Plasma membrane	Dysfunction	Daré et al. 2003
D(3) dopamine receptor	DRD3	1	0	1	16	400	G-protein coupled receptor	Plasma membrane	Dysfunction	Daré et al. 2003
Gamma-aminobutyric acid receptor subunit alpha-1	GABRA1	0	0	0	5	456	Ion channel, receptor	Plasma membrane	Inhibition	Bondy and Agrawal 1980
Gamma-aminobutyric acid receptor subunit beta-1	GABRB1	0	0	1	5	474	Ion channel, receptor	Plasma membrane	Inhibition	Bondy and Agrawal 1980
Muscarinic acetylcholine receptor M3	CHRM3	1	1	0	12	590	Inhibition of adenylate cyclase	Plasma membrane	Inhibition	Abd-Elfattah and Shamoo 1981; Abdallah and Shamoo 1984
Muscarinic acetylcholine receptor M5	CHRM5	2	0	0	17	532	Inhibition of adenylate cyclase	Plasma membrane	Inhibition	Abd-Elfattah and Shamoo 1981; Abdallah and Shamoo 1984
Part C: TRANSPORTERS
Protein	Gene	N° CXXC	N° CXC	N° CC	N° C	N° aa	Function	Localization	Hg Effect	Reference
Calcium-transporting ATPase 1	ATP2B1	0	0	0	17	1,258	Calcium transport	Plasma Membrane	Inhibition	Berg and Miles 1979
Cysteine/glutamate transporter	SLC7A11	0	0	0	7	501	Amino-acid transport	Plasma Membrane	Inhibition	Allen et al. 2001; Fonfría et al. 2005
Part D: OTHER
Protein	Gene	N° CXXC	N° CXC	N° CC	N° C	N° aa	Function	Localization	Hg Effect	Reference
Kelch-like ECH-associated protein 1	KEAP1	0	1	1	27	624	Transcription regulation	Nucleus, Cytoplasm	Modulation	Yoshida et al. 2014
Nuclear factor erythroid 2-related factor 2	NFE2L2	0	0	0	6	605	DNA-binding	Nucleus, Cytoplasm	Up-regulation	Ni et al. 2010
Tubulin beta chain	TUBB	0	1	0	8	444	Nucleotide-binding, microtubule	Cytoskeleton	Oxidation	Kromidas et al. 1990