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. 2020 Apr 28;16(4):e1008592. doi: 10.1371/journal.pgen.1008592

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© 2020 Gao et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — (A)The methylation profile of CpG sites in miR-652-5p. The percentage of methylation in each site was indicated by different colours. Boxes show different methylation patterns in OSCC (n = 18) and corresponding normal tissue samples (n = 18). (B) Hierarchical cluster analysis shows CpG sites methylation in miR-652-5p promoter region in 18 OSCC and normal tissue samples. The colour gradient indicates the methylation level (0% to 100%) of each unit in each sample. (C) The distribution of thirteen analysed CpG units in miR-652-5p promoter. (D) Methylation level of miR-652-5p promoter in OSCC cells treated with 5-Aza-CdR. (E) Quantitative measurement of miR-652-5p level in 5-Aza-CdR-treated cells. Data from triplicate experiments are presented.