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. 2020 Apr 28;15(4):e0227849. doi: 10.1371/journal.pone.0227849

Fig 8. Mitotic DNA damage carried into G1 suggests ongoing repair.

Fig 8

Cells damaged in mitosis where fixed 2 hours post division and immunostained for downstream HR and NHEJ factors as well as for EdU. (A) RAD51 and γH2AX localize to the same area of a G1 cell. (B) EdU and RAD51 co-localize with each other and a phase dark spot of a G1 cell. (C) XRCC4 and γH2AX slightly overlap in a G1 cell. Insets depict a magnified view of damage spots pointed out in arrows. (D) Forty hours post mitosis a cell has a phase dark spot that is surrounded by γH2AX and that co-localizes with EdU. Scale bar = 10μm.