Skip to main content
. 2020 Apr 28;15(4):e0232036. doi: 10.1371/journal.pone.0232036

Fig 3. SQSTM1-NUP214 induce the upregulation of Hoxa and Meis1 genes in hematopoietic cells.

Fig 3

(A) Expression of Hoxa and Meis1 genes determined by qRT-PCR in the bone marrow of leukemic Hoxa9/Meis1 (black, n = 6) or SQSTM1-NUP214 (red, n = 6) mice. Primers mapping to the untranslated regions of Hoxa9 and Meis1 were used to avoid amplifying the transduced proviral sequences in Hoxa9/Meis1 leukemia cells. Shown are means ± SEM. Significance was calculated using the unpaired t test, ** p<0.01. (B) Murine hematopoietic cells from bone marrow (upper panels) or fetal liver (lower panels) were transduced with MSCVpuro (empty vector (EV), white), SQSTM1-NUP214 (SN, red) or SQSTM1-NUP214FGmut (SNFGmut, blue) and selected with puromycin for 3 to 5 days prior to RNA extraction. Transcript levels were determined by qRT-PCR and normalized to the reference gene Gapdh. Shown are mean ± SD from 6 (bone marrow) or 5 (fetal liver) independent experiments. Significance was calculated using the repeated-measures one-way ANOVA (Graphpad Prism). * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.