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. 2020 Apr 28;15(4):e0232428. doi: 10.1371/journal.pone.0232428

Table 2. Von Kossa stain scoring.

Grade
- ± + 2+ 3+
Thoracic aorta
control 5 0 0 0 0
adenine vehicle 3 1 1 2 2
adenine evocalcet 10 0 0 0 0
Abdominal aorta
control 5 0 0 0 0
adenine vehicle 4 1 0 2 2
adenine evocalcet 10 0 0 0 0
Heart
control 5 0 0 0 0
adenine vehicle 6 1 2 0 0
adenine evocalcet 9 1 0 0 0
Kidney
control 5 0 0 0 0
adenine vehicle 0 0 4 5 0
adenine evocalcet 0 2 8 0 0

The data are presented as the number of samples with each von Kossa staining score for each group. Scoring: -: 0% (none), ±: <25% (slight), +: 25–50% (mild), 2+: 50–75% (moderate), 3+: >75% (marked).