Skip to main content
. 2020 Apr 28;15(4):e0229781. doi: 10.1371/journal.pone.0229781

Fig 4. LMN C2 is expressed only in protein form in the oocyte.

Fig 4

A) RT-PCR analysis of the Lmnc2 mRNA expression in the mouse testes (Tes), juvenile ovaries (1-day old mouse pup; JOv) and fully-grown GV oocytes (Oo). GAPDH mRNA was used as a reference gene and reaction without cDNA template was used as a non-template control (NTC), M– 100 bp marker. Black arrowhead depicts endogenous Lmn c2 mRNA. B) Immunoblot detection of expression of LMN C2 in oocytes treated by translational repressor cycloheximide (CHX). GAPDH was used as an endogenous loading control. Representative images from at least three independent experiments are shown. Black arrowhead depicts endogenous LMN C2 protein. C) Quantification of the expression of LMN C2 protein in oocytes treated by CHX. Normalized to GAPDH, presented as mean, ±SD, ns-nonsignificant, t-test.