TABLE 4 -.

Potential miRNA Target Genes Identified with miRWalk and Gene Product Functions Determined by GO Biologic Process

MicroRNA	Target mammalian gene	Gene product function	GO term (accession, ontology)
miR-196–5p	CCL23, chemokine (C-C motif) ligand 23	Chemotactic activity for monocytes, resting T lymphocytes, and neutrophils	GO:0008009, chemokine activity (molecular function)
	RAB29, member RAS oncogene familylike 1	Late endosome-/lysosome-associated small GTPase that regulate endosomal sorting, biogenesis of lysosome and phagocytosis	GO:0017137, Rab GTPase binding (molecular function)
	CTSO, cathepsin O	Proteolytic enzyme involved in cellular protein degradation and turnover	GO:0004197, cysteine-type endopeptidase activity (molecular function)
miR-222–3p	MMP19, matrix metallopeptidase 19	Endopeptidase that degrades various components of the extracellular matrix during development, hemostasis, and pathological conditions	GO:0004222, metalloendopeptidase activity (molecular function)
	PLCE1; phospholipase C, epsilon 1	By generating secondary messengers, this gene plays a role in cell survival, cell growth, actin organization, and T-cell activation	GO:0004435, phosphatidylinositol phospholipase C activity (molecular function)
	AGO3, argonaute RISC catalytic component 3	Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs, which are complementary to them	GO:0035278, miRNA-mediated inhibition of translation (biological process)
miR-99a-5p	ETV3, ETS translocation variant 3	Transcriptional repressor that contributes to growth arrest during terminal macrophage differentiation	GO:0008285, negative regulation of cell proliferation (biological process)
	TRIM71, E3 ubiquitin-protein ligase TRIM71	Binds to miRNAs and associates with AGO2, participates in posttranscriptional repression of transcripts in miRNA-dependent and -independent manner	GO:0061158, 3’-UTR-mediated mRNA destabilization (biological process)
miR-210–3p	E2F3, E2F transcription factor 3	Involved in cell cycle regulation or DNA replication	GO:0008284; positive regulation of cell proliferation (Biological process)
	FGFRL1, fibroblast growth factor receptorlike 1	Negatively regulates cell proliferation	GO:0017134, fibroblast growth factor binding (molecular function)
	SCARA3; scavenger receptor class A, member 3	Protect cells by scavenging oxidative molecules or harmful products of oxidation	GO:0006979, response to oxidative (biological process)
miR-20a-5p	EIF4E, eukaryotic translation initiation factor 4E	Involved in translation by binding to the 7-methylguanosine-containing mRNA cap during the initiation of protein synthesis and facilitates ribosome binding	GO:0003743, translation initiation factor activity (molecular function)
	ITGB6; integrin, beta 6	Integrin alpha-V/beta-6 are involved in cell invasion, cell adhesion; receptor for herpesvirus entry into host cells	GO:0005178; integrin binding (molecular function)
miR-122–5p	VAMP3, vesicle-associated membrane protein 3	SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network	GO:0002479; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent (molecular function)
	XIAP, X-linked inhibitor of apoptosis	Direct caspase inhibitor; modulates inflammatory signaling and immunity, copper homeostasis, mitogenic kinase signaling, cell proliferation	GO:0043027, cysteine-type endopeptidase inhibitor activity involved in apoptotic process (molecular function)

GO, Gene Ontology; GTPase, guanosine triphosphate; MHC, major histocompatibility complex; mNRA, messenger RNA; TAP, transporter associated with antigen processing; UTR, untranslated region.