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. Author manuscript; available in PMC: 2020 May 20.
Published in final edited form as: Nature. 2019 Nov 20;576(7787):459–464. doi: 10.1038/s41586-019-1791-1

Extended Data Figure 3. Biosynthetic gene cluster (BGC) of darobactin in selected bacterial strains.

Extended Data Figure 3

a, The BGC consists of the structural gene darA (colored in blue), darBCD (transporter encoding genes, in grey) and darE (encoding a radical SAM enzyme, in orange). In addition a relE-like gene (black) ORF can be co-located with the BGC at different positions. The BGC can be detected in most Photorhabdus strains in a conserved genetic region. In addition, homologous BGCs (related genes show the identical color code) are in Yersinia, Vibrio and Pseudoalteromonas strains. b, Biosynthetic hypothesis. The propeptide encoded by darA consists of 58 amino acids. The crosslinks are installed on the linear propeptide by DarE. In a next step the leader and tail regions are cleaved off and darobactin is secreted by the ABC transporter DarBCD. c, Amino acid sequence of the propeptide from selected bacterial strains. The darobactin core peptide is highlighted in bold and the amino acids involved in the crosslinking in bold red. The star indicates the stop codon.