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. 2020 Apr 15;12(1):1739825. doi: 10.1080/19420862.2020.1739825

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© 2020 Amgen. Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — (a) CEX-UV profile of a bispecific antibody from two samples that have different degrees of purification before CEX-UV-MS analysis. The UV profile of antibody after only one-column (protein A column) purification is shown in dash line, and the UV profile of antibody after two-column purification is displayed in solid line. (b) CEX-MS profile of the bispecific antibody after only one-column purification. The desired form and impurity were identified based on MS measurement.