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. 2020 Apr 20;147(8):dev184069. doi: 10.1242/dev.184069

Fig. 10.

Fig. 10.

Klc1−/− neurons are unresponsive to CB1R-mediated axonal elongation. (A) Epifluorescence images of Klc1+/+ and Klc1−/− primary cortical neurons transfected with pcDNA3-Cherry and treated for 72 hours with DMSO (vehicle) or CB1R selective agonist ACEA (300 mM). (B) Axonal length quantified in pcDNA-Cherry transfected neurons. Data are mean±s.e.m. Klc1+/+, n=80 (DMSO), n=64 (ACEA); Klc1−/−, n=61 (DMSO), n=61 (ACEA) from three independent experiments. ***P<0.001; two-way ANOVA followed by Bonferroni post-test. (C) Quantification of the number of primary axonal branches. Data indicate median ±25th/75th percentile (box) and 5th/95th percentile (whiskers). (D) Sholl analysis to quantify the average number of dendrite intersections. Data are mean±s.e.m. Klc1+/+, n=84 (DMSO), n=66 (ACEA); Klc1−/−, n=83 (DMSO), n=94 (ACEA) neurons from three independent experiments. Scale bar: 200 μm.