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. 2020 Apr 20;147(8):dev184069. doi: 10.1242/dev.184069

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© 2020. Published by The Company of Biologists Ltd

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Fig. 8. — Dynamic properties of CB1R vesicles are impaired in Klc1^−/− neurons. (A,B) Anterograde (Ante), stationary (Stat) and retrograde (Retro) average run lengths (A) and run length duration (B) obtained from CB1R-eGFP vesicle single trajectories using MATLAB. Data are mean±s.e.m. Klc1^+/+, n=1499 (ante), n=1487 (retro), n=1897 (stat); Klc1^−/−, n=595 (ante), n=638 (retro), n=911 (stat). *P<0.01, **P<0.001, ***P<0.0001; Student's t-test. (C) Distribution of segmental velocities for anterograde and retrograde CB1R-eGFP vesicle trajectories. Insets show relative frequency distribution of segmental velocities. Data show probability density function analysis of pooled segmental velocities. Klc1^+/+, n=1821 (ante), n=1729 (retro); Klc1^−/−, n=482 (ante), n=556 (retro). ***P<0.00001; Kolmogorov–Smirnov test.