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. 2020 Apr 21;9:e54056. doi: 10.7554/eLife.54056

Figure 1. Reduced leakage from CNV lesions in KdrY949F/Y949F retinas at D14.

Figure 1.

(A) Representative CNV lesions imaged from whole mount choroid tissue, collected at day (D) 14 after laser injury, from KdrY949F/Y949F and Kdr+/+ littermates, immunostained for isolectin B4 (IB4). Scale bar = 100 µm. Dotted red line highlights the extent of lesion formation. (B) Quantification of average lesion size at D14 after injury. n = 60–67 lesions per group from 9 to 11 mice per group. ns = not significant p=0.6882. (C) Representative images of D14 lesions from KdrY949F/Y949F and Kdr+/+ littermates immunostained for IB4 (red), showing accumulation of tail-vein injected, fluorescent 100 nm microspheres (white) in the tissue around the lesions. Insets enlarged (right) with microspheres shown as black dots on white background. Scale bar = 100 µm. Inset scale bar = 25 µm. Arrows point to areas of microsphere accumulation. (D) Quantification of the average area of accumulated microspheres per image after 2 min of circulation. n = 35–74 lesions per group from 7 to 14 mice per group. ***p<0.001 p=0.0006.

Figure 1—source data 1. Text file containing the ImageJ macro code used to quantify microsphere accumulation in CNV experiments.
Figure 1—source data 2. Excel file containing the collected CNV lesion size and microsphere area.