Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | KdrY949F/Y949F | DOI: 10.1038/ncomms11017 | C57Bl/6 background | |
| Strain; strain background (Mus musculus) | Sh2d2afl/fl; Cdh5-CreERT2 | DOI: 10.1126/scisignal.aad9256 | C57Bl/6 background | |
| Strain; strain background (Mus musculus) | mT/mG | DOI: 10.1002/dvg.20335 |
C57Bl/6 background | |
| Strain; strain background (Mus musculus) | VEC-Y685F | DOI: 10.1038/ni.2824 | C57Bl/6 background | |
| Strain; strain background (Mus musculus) | VEC-WT | DOI: 10.1038/ni.2824 | C57Bl/6 background | |
| Antibody | Rat anti-VE-cadherin | BD Biosciences | Catalogue no: 555289 RRID:AB_395707 |
(1:100) |
| Antibody | Rabbit anti-VE-cadherin pY658 | DOI: 10.1038/ncomms2199 | (1:50) | |
| Antibody | Rabbit anti-VE-cadherin pY688 | DOI: 10.1038/ncomms2199 | (1:50) | |
| Antibody | Rabbit anti-phospho-Src (Tyr418) | Thermo Fisher Scientific | Catalogue no: 44–660G RRID:AB_1500523 |
(1:100) |
| Antibody | Goat anti-CD45 | BD Biosciences | Catalogue no: 553076 RRID:AB_394606 |
(1:300) |
| Antibody | Rat anti-CD68 | BioRad Laboratories | Catalogue no: MCA1957 RRID:AB_322219 |
(1:300) |
| Antibody | Donkey anti-Rat | Thermo Fisher Scientific |
Catalogue no: A-21208 RRID:AB_141709 |
(1:500) |
| Antibody | Donkey anti-Rabbit | Thermo Fisher Scientific | Catalogue no: A-31572 RRID:AB_162543 |
(1:500) |
| Antibody | Donkey anti-Goat | Thermo Fisher Scientific | Catalogue no: A-21432 RRID:AB_2535853 |
(1:500) |
| Software | ImageJ | NIH, Bethesda, MD, USA |
RRID:SCR_003070 | |
| Software | GraphPad Prism | GraphPad | RRID:SCR_002798 | |
| Other | Alexa Fluor 488-Isolectin B4 | Thermo Fisher Scientific | Catalogue no I21411 RRID:AB_2314662 |
(1:500) |
| Other | Alexa Fluor 594-Isolectin B4 | Thermo Fisher Scientific | Cataolgue no: I21413 RRID:AB_2313921 |
(1:500) |
| Other | Alexa Fluor 647-Isolectin B4 | Thermo Fisher Scientific | Catalogue no: I32450 RRID:SCR_014365 |
(1:500) |
| Other | Fluoro-Max Dyed Blue Aqueous Fluorescent Particles | Thermo Fisher Scientific | Catalogue no: B0100 | |
| Other | Fluoro-Max Dyed Green Aqueous Fluorescent Particles | Thermo Fisher Scientific | Catalogue no: G25 |