Figure 2. Expression of neocentromeric genes.
Expression of the neocentromeric genes was assessed by qPCR for all cenΔ mutants and expression is shown as Log2ΔΔCt. For cen10∆-A, cen10∆-B and cen10∆-C, two genes were selected from each neocentromeric region, all other cen∆ mutants are represented by one gene spanned by CENP-A. cen10∆-B has only one CENP-A-enriched region, and in this case, the genes located within primary peak of cen10∆-A and cen10∆-C served as controls. The qPCRs of cen10∆ mutants are normalized with a housekeeping gene located on chromosome 10. The qPCRs of cen9∆ mutants are normalized with actin. Error bars show standard deviation.