Figure 3. cen10∆ mutant strains have reduced fitness compared to the wild-type strain.

(A) Six out of seven cen10∆ mutants had a longer doubling time and slower growth than the wild-type strain. In contrast cen10∆-E grows similarly to the wild type. Error bars show standard deviation. (B) Doubling times and fold change compared to wild type are shown. (C) Competition assays with the wild type and cen9∆ and cen10∆ mutant strains. Mixed cultures (1:1) were grown overnight and plated with and without selection agents. After four days, colonies were counted and the percentage of cenΔ mutants (black) and wild type (grey) in each culture was plotted. As a control (C) a wild-type strain with a NAT marker was mixed with the wild type.

Figure 3—figure supplement 1. cen10∆ mutants with chromosomal fusion have a wild-type growth rate.

The doubling times of large and small colonies derived from cen10∆ mutants at 30°C were determined. Large colonies had a growth rate similar to the wild-type strain, while small colonies and the initial cen10∆ mutants had a similar growth rate with one another but that was slower than the wild-type strain and the derived large colonies. (A) Growth curves for cen10∆-A-derived isolates and the wild-type strain are shown. (B) Growth curves for cen10∆-B-derived isolates and the wild-type strain are shown.