Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent Cryptococcus deuterogattii |
R265 | This study | R265 expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-A | This study | R265 centromere 10 deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-B | This study | R265 centromere 10 deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-C | This study | R265 centromere 10 deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-D | This study | R265 centromere 10 deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-E | This study | R265 centromere 10 deletion mutant with expressingmCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-F | This study | R265 centromere 10 deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-G | This study | R265 centromere 10 deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-A-S1 | This study | Small colony derived from R265 centromere 10A deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-A-L1 | This study | Large colony derived from R265 centromere 10A deletion mutant with expressingmCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-B-S1 | This study | Small colony derived from R265 centromere 10B deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-B-S2 | This paper | Small colony derived from R265 centromere 10B deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-B-S3 | This study | Small colony derived from R265 centromere 10B deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-B-L1 | This study | Large colony derived from R265 centromere 10B deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-B-L2 | This study | Large colony derived from R265 centromere 10B deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-C-S1 | This study | Small colony derived from R265 centromere 10C deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-C-S2 | This study | Small colony derived from R265 centromere 10C deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-C-S3 | This study | Small colony derived from R265 centromere 10C deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-C-L1 | This study | Large colony derived from R265 centromere 10C deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-C-L2 | This study | Large colony derived from R265 centromere 10C deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-E-S1 | This study | Small colony derived from R265 centromere 10E deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-E-S2 | This study | Small colony derived from R265 centromere 10E deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-E-S3 | This study | Small colony derived from R265 centromere 10E deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-E-L1 | This study | Large colony derived from R265 centromere 10E deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-E-L2 | This study | Large colony derived from R265 centromere 10E deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen9△-A | This study | R265 centromere 9 deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen9△-B | This study | R265 centromere 9 deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen9△-C | This study | R265 centromere 9 deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen9△-D | This study | R265 centromere 9 deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen9△-E | This study | R265 centromere 9 deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen9△-F | This study | R265 centromere 9 deletion mutant with expressing mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
R265 MIS12 | This study | R265 expressing GFP-MIS12 and mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-A MIS12 | This study | R265 Centromere 10 mutant with expressing GFP-MIS12 and mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-B MIS12 | This study | R265 Centromere 10 mutant with expressing GFP-MIS12 and mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-C MIS12 | This study | R265 Centromere 10 mutant with expressing GFP-MIS12 and mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-D MIS12 | This study | R265 Centromere 10 mutant with expressing GFP-MIS12 and mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen10△-E MIS12 | This study | R265 Centromere 10 mutant with expressing GFP-MIS12 and mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
R265 CENPC | This study | R265 with expressing GFP-CENPC and mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen9△-A CENPC | This study | R265 Centromere 9 mutant with expressing GFP-CENPC and mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen9△-B CENPC | This study | R265 Centromere 9 mutant with expressing GFP-CENPC andmCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen9△-C CENPC | This study | R265 Centromere 9 mutant with expressing GFP-CENPC andmCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen9△-D CENPC | This study | R265 Centromere 9 mutant with expressing GFP-CENPC and mCherry-CENPA | |
| Genetic reagent Cryptococcus deuterogattii |
cen9△-E CENPC | This study | R265 Centromere 9 mutant with expressing GFP-CENPC and mCherry-CENPA | |
| Antibody | Anti-mCherry antibody(Rabbit polyclonal) | Abcam | Cat. no. ab183628 |
ChIP (1/5000) |
| Antibody | Anti-GFP (Rabbit polyclonal) antibody | Abcam | Cat. no. ab290 |
ChIP (5 µg for 1 µg of chromatin) |
| Other | Dynabeads Protein A for Immunoprecipitation | Invitrogen | Cat. no. 10001D |
ChIP (20 µl per500 µl fraction) |
| Software, algorithm | Bowtie2 | Langmead, 2010 | ||
| Software, algorithm | Spades | Bankevich et al., 2012 | ||
| Software, algorithm | IGV | Thorvaldsdóttir et al., 2013 | ||
| Software, algorithm | HISAT2 | Pertea et al., 2016 | ||
| Sequence-based reagent | List of primers used in this study | Sigma | In Supplementary file 4 |