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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: J Immunol Methods. 2020 Mar 2;480:112766. doi: 10.1016/j.jim.2020.112766

Figure 7. MT4-CCR5-Luc cells can be used to estimate ADCC against viruses with primary Envs of interest.

Figure 7.

(A) MT4-CCR5-Luc cells were infected with viruses representing the circulating strains present in an infected mother. Percent ADCC (y-axis) was determined after incubating infected cells with NK cells and a serial dilution (x-axis) of either maternal plasma (MpL) or infant plasma (IpL). Top dilution was 1:50 of plasma. Bars show SEM from three independent experiments with each condition tested in triplicate. (B) Heterologous ADCC responses of maternal plasma against BJOX2000-infected cells (y-axis) were plotted against neutralization responses (x-axis) from the same samples against the same virus. Dashed line at y = 25 denotes threshold for nonspecific cytotoxicity.