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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: FEBS Lett. 2019 Dec 31;594(8):1307–1318. doi: 10.1002/1873-3468.13721

Figure 1. PhAXA identifies putative 4E-BP1 kinases in cells released from a nocodazole-arrest.

Figure 1.

(A) Log2 fold change of LFQ ratios for the 3XFLAG-4E-BP1 (T46) probe relative to appropriate controls. ‘+’ refers to samples treated with crosslinker, while ‘-‘ refers to ATP only controls. Fold-changes were capped at 100 for this graph. Dotted lines represent 4-fold enrichment. (B) Table of all protein kinases identified by 3 or more peptide-spectrum-matches (PSMs) in at least one sample. (C) CDK4 is enriched from lysate generated from nocodazole arrested cells expressing 3XFLAG-4E-BP1 WT, T37C and T46C mutants. A representative input is included to show the mass shift of CDK4 upon crosslinking to 4E-BP1.