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. 2020 Mar 17;122(9):1378–1390. doi: 10.1038/s41416-020-0792-z

Fig. 4. Self-renewal potential of the CSC-like cells.

Fig. 4

a Representative images for adherent culture of Huh7, miPS-Huh7cm and miPS-Huh7cmP1 cells. Scale bars represent 100 μm. b FACS analysis shows GFP population in miPS-Huh7cm and miPS-Huh7cmP1 cells. c Sphere-formation assay in serum-free medium shows spherogenic potential of both miPS-Huh7cm and miPS-Huh7cmP1 cells compared to Huh7 cells. Scale bars: 50 μm. d Number of spheres formed from miPS-Huh7cmP1 cells compared to miPS-Huh7cm and Huh7 cells. e Extreme limiting dilution assay assessment of the limiting dilution sphere-forming potential of miPS-Huh7cm and miPS-huh7cmP1 cells. f Immunofluorescence staining for Nanog and Oct 3/4 in tumour spheroid derived from miPS-Huh7cm and miPS-Huh7cmP1 cells. Scale bars represent 64 μm.