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. 2020 Apr 28;10:7177. doi: 10.1038/s41598-020-64204-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Speckle Image Processing Pipeline. A commercial fundus camera (Phoenix Micron IV) was modified by replacing a xenon light source with a 640 nm laser light source (A). Acquired speckle images could be processed via a temporal processing scheme (B) which results in a single contrast image with high spatial resolution (C,D) or a spatial processing scheme (E) in which several spatially processed contrast images with low spatial resolution but greater temporal resolution (F,G) can be averaged to generate a single noise reduced contrast image with enhanced spatial resolution (H,I). Scale bars: B–F 150 μm.