Fig. 4.

Fine mapping of qAWNL2. a Molecular markers between RM5707 and RM6380 on chromosome 2 were used to detect recombinants near the qAWNL2 locus. b Graphical genotypes of BC₃F₅ lines derived from five critical recombinant BC₃F₄ plants (nos. 146–82, − 211, − 228, − 137 and − 17) in the candidate region of qAWNL2. C, W and R: Homozygous IR36, W630 and recombinant chromosomes, respectively. Awn lengths (means ± SD, n = 5) of the 5th spikelets of the BC₃F₅ lines are shown on right. ** and n.s.: significant at P < 0.01 and not significant, respectively. The qAWNL2 locus was estimated in the 157.4-kb region between RM13335 and RM13349