Fig. 4. Presence of constitutively active Chk1 inhibits the accumulation of Cdh1 through S/G2 phase.
a 293T cells were transfected with Myc-Chk1L449R, treated with HU (2 mM) for 20 h and released into fresh media. At the indicated time points, cells were harvested to prepare the whole-cell lysates for immunoblot analysis. b Quantification of band intensities as in (1e). The intensities of Cdh1 were normalized to actin and then further normalized to vector control. Data are represented as mean ± SD, *p < 0.05 by unpaired t-test, n = 4. c Cell-cycle progression was monitored by flow cytometry. The experimental set up was as described as in (a).