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. 2020 Apr 28;11(4):298. doi: 10.1038/s41419-020-2493-1

Fig. 6. Chk1-mediated phosphorylation of Cdh1 destabilizes the Cdh1–APC/C interaction.

Fig. 6

a In vitro binding of Cdh1 to the APC/C in G1/S extracts from HU-arrested HeLa cells with or without Cdki (purvalanol) or Chk1i. Immunoblots represent both immunoprecipitated HA-Cdh1 and the APC/C subunit Cdc27. b In vitro binding of wild-type or mutant Cdh1 proteins to the APC/C in G1/S extracts from HU arrested HeLa cells with or without Chk1i. Immunoblots represent both immunoprecipitated HA-Cdh1 constructs and the APC/C subunit Cdc27 with the relative inputs of the Cdh1 constructs (n = 3). Data represent mean and SEM. Significance was determined by one-way ANOVA with Holm-Sidak post-test, *p < 0.05.