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. 2020 Apr 28;10:7163. doi: 10.1038/s41598-020-64145-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of enzymatic activity: (a) Muralytic activity of ElyA1 was determined by spotting ElyA1 and endolysin buffer as a negative control in an overlay of two Gram-negative Acinetobacter baumannii clinical isolates, MAR001 and PAU002; (b) pH range and (c) temperature range were determined by the specific activity, measured as the difference in optical density of the culture per µg of enzyme and minute.