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. 2020 Apr 28;10:7173. doi: 10.1038/s41598-020-64041-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A–L) Light micrographs showing portions of the capsules adjacent to the opening of the tubes implanted in the subcutaneous for 7 and 60 days. (A,C,E,G,I–L) The sections were subjected to von Kossa histochemical method and counterstained with picrosirius. von Kossa-positive structures (black colour) are seen dispersed by capsules of GFB (A,C) and MTAF (E,G) groups. von Kossa-positive structures are not observed in the EF (I,J) and CG groups (K,L). (B,D,F,H) Light micrographs showing unstained sections analyzed under polarized light. Note birefringent structures in the corresponding regions of the capsules shown in the sections submitted to the von Kossa method. T, space of the tube implanted; Bars: 65 µm.