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. 2020 Apr 28;11:2055. doi: 10.1038/s41467-020-15954-x

Fig. 5. Unrepaired DSBs persist and activate somatic-like DDR in Brme1−⁄− mice.

Fig. 5

a–d Immunostaining of spermatocytes. RPA2 a, γH2AX b, RAD51 c, and DMC1 d were stained. Graph: the number of foci associated with the chromosome axes. Asterisks in b: sex chromosomes. Red bars: mean value. n shows the analyzed spermatocyte number pooled from three mice for each genotype. e–g Immunostaining of Brme1−⁄− spermatocytes. RAD51 e and DMC1 g were stained. A chromosome in e is magnified in f. Asterisks: sex chromosomes. h Schematic of DDR pathway activation in WT and Brme1−⁄− males. Unrepaired DSBs persist in Brme1−⁄− males owing to the low activation of meiotic recombinases in early prophase I, which subsequently activates somatic-like DDR in late prophase I. All analyses used two-tailed t-tests. ns: not significant. ****p < 0.0001. Leptotene (L), zygotene (Z), early-pachytene (EP), late-pachytene (LP), diplotene (D). Scale bars: 5 μm or 1 μm in the magnified panel. Source data are provided as a Source Data file.